sterile inoculated spores
inoculating sterile spores some people think that inoculation is a complicated process, but you don’t. I don’t need a hood
or any sophisticated equipment for inoculating jars. Just take a little
respect for the task and the level of cleanliness it requires. Really
much more time and care is devoted to preparing the pots themselves than to
in fact, by inoculating them.
Start by choosing a work surface that you feel comfortable working on.
A counter or kitchen table works well. The most important thing is
that it is a clean area, free of dirt, dust and direct airflow from a
vent. It also won’t hurt to take a shower and put on clean clothes.
How to inoculate spores
The inoculation of your pots is the main step where contamination is possible, and therefore
should be carried out in as clean an environment as possible. If the room you are in
work is pretty clean (most are not), you can get away with inoculating
them in the open air. The needle of the syringe, if it is not absolutely sterile, can carry
bacteria and other mold spores in your cake, contaminating and ruining
the cake. The open air in your home is filled with millions of microscopic molds
spores and bacteria, so even one of them falls on your spore needle
The syringe can infect a jar. Wash your hands and face with antibacterial soap. To carry
clean clothes. Anything near the syringe and jars could contaminate your
cakes if they are not clean. There are two methods of injecting your spores that help
eliminate any possible contaminants from the air.
1. Glove box method
If you are concerned about infertility, a good way to address this is to have a
“glove box” means a closed, semi-sealed box with holes for gloves to pass through and
a transparent top. A cheap, half-decent model can be built for just a few people.
stuff worth a dollar. All you need is a large cardboard box, some tape, and saran wrap.
wrapping paper to cover the top of the box and a pair of new, unused dishwashing gloves.
Stick some tape on top and cut two holes big enough for your arms in the
sides. Disinfect the gloves and the inside of the box with Lysol disinfectant spray.
A small door can be cut into the side of the box to collect the syringe and jars.
in the box, or they can be passed through one of the arm holes (if you choose not to).
attach the gloves to the holes.