Mushroom Substrate Kit Large Sterile substrate for growing mushrooms
The sterilized mushroom substrate can be considered a breeding ground for a plant. This sterilized substrate must be inoculated with our liquid cultures obtained from of spores.
Contains the mushroom substrate kit
- Container with 1200 cc/ml of sterile substrate mix
- Liquid Culture 10 ml
- Injection port
- Fruiting bag with filter
- Alcohol exchange
The substrate contains:
Sterilized substrate with moisture content: 60–65% A blend of whole oats, vermiculite, perlite, and gypsum
Recommended products to use
- Spore syringe
- Spore Vial
- Facial mask
- Hand sanitizer gel
Always store your substrate kit in a clean refrigerator! We recommend using the substrate kit as soon as possible. The kit can be stored in the refrigerator for up to 6 weeks.
How to use a mushroom substrate kit: instructions
Phase 1: Prepare the liquid culture
Wash your hands and clean the work area!
Shake the spore bottle or syringe well to make sure all the spores float.
- Disinfect the liquid culture injection site and spore vial or syringe with the alcohol swab.
- Inject 2 ml of spore solution into the liquid culture
Shake the inoculated liquid culture vigorously once a day, every day, for 10 days. Store the inoculated liquid culture in a place between 23 and 27 degrees Celsius. The liquid culture is ready when you see a cloud floating in the suspension. It’s mycelium…
Your liquid culture is ready; it is called the master culture. If used and stored correctly, you can have a lot of mycelium from just a few spores.
- If the liquid is cloudy, it means there is contamination and the culture is unusable.
- If you do not use the liquid culture for 10 days, store it in the refrigerator
Phase 2: Inoculation
- Wash your hands and clean your workspace
- Clean the injection site of the sterilized substrate and liquid culture
- Remove 10 ml (1200 CC kit) or 10 ml (2100 CC kit) from the liquid culture
- Inject the spore suspension through the injection site of the kit
After inoculation, turn and shake the box 360 degrees in all directions
- store the kit in a dark place with a temperature between 23° and 27°C
After inoculation, you will see mycelium forming. A white mushroom will gradually fill the sterilized substrate. This may take up to 2–3 weeks, depending on the temperature.
Phase 3 – Fruit and spore collection
~ Please keep in mind that fruit mushrooms may be illegal in your country. Always check your local laws before taking this step. ~
When the substrate is fully colonized, it is time to grow the fruits to collect mushroom spores or mushroom tissues for further research or cultivation.