Sterile magic mushroom substrate for p. Cubensis Large

Description

Mushroom Substrate Kit Large Sterile substrate for growing mushrooms

The sterilized mushroom substrate can be considered a breeding ground for a plant. This sterilized substrate must be inoculated with our liquid cultures obtained from of spores.

Contains the mushroom substrate kit

• Container with 1200 cc/ml of sterile substrate mix
• Liquid Culture 10 ml
• Injection port
• Fruiting bag with filter
• Alcohol exchange
• Clip

The substrate contains:
Sterilized substrate with moisture content: 60–65% A blend of whole oats, vermiculite, perlite, and gypsum

Recommended products to use

• Spore syringe
• Spore Vial
• Facial mask
• Gloves
• Lighter
• Hand sanitizer gel

Storage

Always store your substrate kit in a clean refrigerator! We recommend using the substrate kit as soon as possible. The kit can be stored in the refrigerator for up to 6 weeks.

How to use a mushroom substrate kit: instructions

Phase 1: Prepare the liquid culture

Wash your hands and clean the work area!

Shake the spore bottle or syringe well to make sure all the spores float.
1. Disinfect the liquid culture injection site and spore vial or syringe with the alcohol swab.
2. Inject 2 ml of spore solution into the liquid culture

Shake the inoculated liquid culture vigorously once a day, every day, for 10 days. Store the inoculated liquid culture in a place between 23 and 27 degrees Celsius. The liquid culture is ready when you see a cloud floating in the suspension. It’s mycelium…

Your liquid culture is ready; it is called the master culture. If used and stored correctly, you can have a lot of mycelium from just a few spores.

Important!

• If the liquid is cloudy, it means there is contamination and the culture is unusable.
• If you do not use the liquid culture for 10 days, store it in the refrigerator

Phase 2: Inoculation

Step 1

• Wash your hands and clean your workspace
• Clean the injection site of the sterilized substrate and liquid culture

Step 2

• Remove 10 ml (1200 CC kit) or 10 ml (2100 CC kit) from the liquid culture

• Inject the spore suspension through the injection site of the kit

Step 3

After inoculation, turn and shake the box 360 degrees in all directions
• store the kit in a dark place with a temperature between 23° and 27°C

Result:

After inoculation, you will see mycelium forming. A white mushroom will gradually fill the sterilized substrate. This may take up to 2–3 weeks, depending on the temperature.

Phase 3 – Fruit and spore collection

~ Please keep in mind that fruit mushrooms may be illegal in your country. Always check your local laws before taking this step. ~

When the substrate is fully colonized, it is time to grow the fruits to collect mushroom spores or mushroom tissues for further research or cultivation.